Ilex hainanensis Merr is the national minority folk herbal medicine in the Guangxi Zhuang Autonomous Region, and its effects of blood invigorate and pulse promotion can provide potential therapeutic approaches to treatment cardiovascular diseases. The aim of this paper is to observe the effect of different extracts from Ilex hainanensis Merr (IHE) on human platelet aggregation induced by arachidonic acid sodium salt (AA), adenosine diphosphate (ADP), and epinephrine hydrochlaride (ADR), respectively, in, vitro. In this study, aspirin was used as positive control drug to establish the in vitro anti-platelet aggregation model and platelet aggregation rate was measured by Born's method. Platelet-rich plasma was collected from healthy donors and induced by AA, ADP and ADR, respectively and the model was applied to study the effect of different IHE on the platelet aggregation. The results showed that aspirin can significantly inhibit platelet aggregation induced by AA, ADP and ADR in vitro, respectively, but the IHE did not show inhibiting effect on the human platelet aggregation significantly. However, the IHE negative results can provide solid experimental evidence for improving drug quality standard.