Residential College | false |
Status | 已發表Published |
First Synthesis of an Oridonin-Conjugated Iridium(III) Complex for the Intracellular Tracking of NF-κB in Living Cells | |
Wang W.2; Yang C.4![]() ![]() ![]() ![]() | |
2017-04-06 | |
Source Publication | Chemistry - A European Journal
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ISSN | 15213765 09476539 |
Volume | 23Issue:20Pages:4929-4935 |
Abstract | NF-κB is a critical transcription factor that plays an important role in mediating inflammation, the immune response, and cell proliferation. The activation of NF-κB leads to an enhancement of proinflammatory mediator expression, which is implicated in the pathogenesis of a variety of diseases. Therefore, methods that allow the intracellular tracking of NF-κB are particularly attractive because they can provide information regarding the pathways or stimulation responses that are involved in the activation of NF-κB. In this work, we report a novel platform to track intracellular NF-κB by employing the conjugated iridium(III) complex 1, which was synthesized through the unique combination of a luminescent iridium(III) moiety with the natural product oridonin. Experiments conducted with p50 knockdown cells revealed that complex 1 could detect the p50 subunit of NF-κB in cellulo. Furthermore, complex 1 tracked NF-κB translocation induced by tumor necrosis factor-α (TNF-α) as a model stimulus, without affecting the translocation process itself. To the best of our knowledge, complex 1 is the first metal-based compound that has been reported to be capable of monitoring intracellular NF-κB in living cells. |
Keyword | Cell Imaging Iridium(Iii) Complex Natural Products Nf-κb |
DOI | 10.1002/chem.201700770 |
URL | View the original |
Indexed By | SCIE |
WOS Research Area | Chemistry |
WOS Subject | Chemistry, Multidisciplinary |
WOS ID | WOS:000399327100026 |
Scopus ID | 2-s2.0-85016249891 |
Fulltext Access | |
Citation statistics | |
Document Type | Journal article |
Collection | DEPARTMENT OF MATHEMATICS Institute of Chinese Medical Sciences |
Affiliation | 1.Hunan University 2.Hong Kong Baptist University 3.University of Florida 4.University of Macau |
Recommended Citation GB/T 7714 | Wang W.,Yang C.,Lin S.,et al. First Synthesis of an Oridonin-Conjugated Iridium(III) Complex for the Intracellular Tracking of NF-κB in Living Cells[J]. Chemistry - A European Journal, 2017, 23(20), 4929-4935. |
APA | Wang W.., Yang C.., Lin S.., Vellaisamy K.., Li G.., Tan W.., Leung C.-H.., & Ma D.-L. (2017). First Synthesis of an Oridonin-Conjugated Iridium(III) Complex for the Intracellular Tracking of NF-κB in Living Cells. Chemistry - A European Journal, 23(20), 4929-4935. |
MLA | Wang W.,et al."First Synthesis of an Oridonin-Conjugated Iridium(III) Complex for the Intracellular Tracking of NF-κB in Living Cells".Chemistry - A European Journal 23.20(2017):4929-4935. |
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