| Residential College | false |
| Status | 已發表Published |
| Reconstitution of chemotactic peptide-induced nicotinamide adenine dinucleotide phosphate (reduced) oxidase activation in transgenic COS-phox cells | |
| He R.1; Nanamori M.1; Sang H.1; Yin H.1; Dinauer M.C.3; Ye R.D.1 | |
| 2004-12-15 | |
| Source Publication | Journal of Immunology
 |
| ISSN | 00221767 |
| Volume | 173Issue:12Pages:7462-7470 |
| Abstract | A whole-cell-based reconstitution system was developed to study the signaling mechanisms underlying chemoattractant-induced activation of NADPH oxidase. This system takes advantage of the lack of formyl peptide receptor-mediated response in COS-phox cells expressing gp91, p22, p67, and p47, which respond to phorbol ester and arachidonic acid with O production. By exogenous expression of signaling molecules enriched in neutrophils, we have identified several critical components for fMLP-induced NADPH oxidase activation. Expression of PKCδ, but not PKCα, -βII, and -ζ, is necessary for the COS-phox cells to respond to fMLP. A role of PKCδ in neutrophil NADPH oxidase was confirmed based on the ability of fMLP to induce PKCδ translocation and the sensitivity of fMLP-induced O production to rottlerin, a PKCδ-selective inhibitor. Optimal reconstitution also requires phospholipase C-β2 and PI3K-γ. We found that formyl peptide receptor could use the endogenous Rac1 as well as exogenous Rac1 and Rac2 for NADPH oxidase activation. Exogenous expression of p40 potentiated fMLP-induced O production and raised the level of O in unstimulated cells. Collectively, these results provide first direct evidence for reconstituting fMLP-induced O production in a nonhemopoietic cell line, and demonstrate the requirement of multiple signaling components for optimal activation of NADPH oxidase by a chemoattractant. |
| URL | View the original |
| Language | 英語English |
| Fulltext Access | |
| Document Type | Journal article |
| Collection | University of Macau |
| Affiliation | 1.University of Illinois College of Medicine 2.University of Illinois at Chicago 3.Indiana University School of Medicine Indianapolis |
| Recommended Citation GB/T 7714 | He R.,Nanamori M.,Sang H.,et al. Reconstitution of chemotactic peptide-induced nicotinamide adenine dinucleotide phosphate (reduced) oxidase activation in transgenic COS-phox cells[J]. Journal of Immunology, 2004, 173(12), 7462-7470. |
| APA | He R.., Nanamori M.., Sang H.., Yin H.., Dinauer M.C.., & Ye R.D. (2004). Reconstitution of chemotactic peptide-induced nicotinamide adenine dinucleotide phosphate (reduced) oxidase activation in transgenic COS-phox cells. Journal of Immunology, 173(12), 7462-7470. |
| MLA | He R.,et al."Reconstitution of chemotactic peptide-induced nicotinamide adenine dinucleotide phosphate (reduced) oxidase activation in transgenic COS-phox cells".Journal of Immunology 173.12(2004):7462-7470. |
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