Residential College | false |
Status | 已發表Published |
Expression of Smad4 Gene in Pichia pastoris and Identification of the Protein | |
Liu Y.-P.1; Yang X.3; Xu X.-L.2; Deng J.-X.3; Cheng X.3; Huang C.-F.3 | |
2000-12-01 | |
Source Publication | Acta Biochimica et Biophysica Sinica |
ISSN | 05829879 |
Volume | 32Issue:3 |
Abstract | Smad4 is a novel tumor suppressor gene which is mutated or deleted in about 50% of pancreatic carcinoma and 30% of colon cancer. SMAD4 was expressed in Pichia pastoris and was characterized. The molecular weight of the expression product was shown to be about 67 kD, and 13 amino acids in N terminal were determined and were identical with the putative sequence from Smad4 cDNA, and it was able to specifically react with the antibody against SMAD4. |
Keyword | Pichia pastoris Smad4 Yeast expression |
URL | View the original |
Language | 英語English |
Fulltext Access | |
Document Type | Journal article |
Collection | University of Macau |
Affiliation | 1.Military Medical Institute of Guangzhou 2.National Institute of Diabetes and Digestive and Kidney Diseases 3.Academy of Military Medical Science China |
Recommended Citation GB/T 7714 | Liu Y.-P.,Yang X.,Xu X.-L.,et al. Expression of Smad4 Gene in Pichia pastoris and Identification of the Protein[J]. Acta Biochimica et Biophysica Sinica, 2000, 32(3). |
APA | Liu Y.-P.., Yang X.., Xu X.-L.., Deng J.-X.., Cheng X.., & Huang C.-F. (2000). Expression of Smad4 Gene in Pichia pastoris and Identification of the Protein. Acta Biochimica et Biophysica Sinica, 32(3). |
MLA | Liu Y.-P.,et al."Expression of Smad4 Gene in Pichia pastoris and Identification of the Protein".Acta Biochimica et Biophysica Sinica 32.3(2000). |
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