Residential College | false |
Status | 已發表Published |
GLUT12 promotes prostate cancer cell growth and is regulated by androgens and CaMKK2 signaling | |
White, Mark A.1,2; Tsouko, Efrosini1,2; Lin, Chenchu3,4; Rajapakshe, Kimal5; Spencer, Jeffrey M.1,2; Wilkenfeld, Sandi R.3,4; Vakili, Sheiva S.1,2; Pulliam, Thomas L.1,2,3; Awad, Dominik3,4; Nikolos, Fotis5; Katreddy, Rajasekhara Reddy2; Kaipparettu, Benny Abraham6,7; Sreekumar, Arun5,7; Zhang, Xiaoliu1,2; Cheung, Edwin8,9; Coarfa, Cristian5; Frigo, Daniel E.1,2,3,10,11 | |
2018-04 | |
Source Publication | ENDOCRINE-RELATED CANCER |
ISSN | 1351-0088 |
Volume | 25Issue:4Pages:453-469 |
Abstract | Despite altered metabolism being an accepted hallmark of cancer, it is still not completely understood which signaling pathways regulate these processes. Given the central role of androgen receptor (AR) signaling in prostate cancer, we hypothesized that AR could promote prostate cancer cell growth in part through increasing glucose uptake via the expression of distinct glucose transporters. Here, we determined that AR directly increased the expression of SLC2A12, the gene that encodes the glucose transporter GLUT12. In support of these findings, gene signatures of AR activity correlated with SLC2A12 expression in multiple clinical cohorts. Functionally, GLUT12 was required for maximal androgen-mediated glucose uptake and cell growth in LNCaP and VCaP cells. Knockdown of GLUT12 also decreased the growth of C4-2, 22Rv1 and AR-negative PC-3 cells. This latter observation corresponded with a significant reduction in glucose uptake, indicating that additional signaling mechanisms could augment GLUT12 function in an AR-independent manner. Interestingly, GLUT12 trafficking to the plasma membrane was modulated by calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2)-5'-AMP-activated protein kinase (AMPK) signaling, a pathway we previously demonstrated to be a downstream effector of AR. Inhibition of CaMKK2-AMPK signaling decreased GLUT12 translocation to the plasma membrane by inhibiting the phosphorylation of TBC1D4, a known regulator of glucose transport. Further, AR increased TBC1D4 expression. Correspondingly, expression of TBC1D4 correlated with AR activity in prostate cancer patient samples. Taken together, these data demonstrate that prostate cancer cells can increase the functional levels of GLUT12 through multiple mechanisms to promote glucose uptake and subsequent cell growth. |
Keyword | Glut12 Slc2a12 Glucose Metabolism Androgen Receptor (Ar) Calcium/calmodulin-dependent Protein Kinase Kinase 2 (Camkk2) 5 '-amp-activated Protein Kinase (Ampk) Prostate Cancer |
DOI | 10.1530/ERC-17-0051 |
URL | View the original |
Indexed By | SCIE |
Language | 英語English |
WOS Research Area | Oncology ; Endocrinology & Metabolism |
WOS Subject | Oncology ; Endocrinology & Metabolism |
WOS ID | WOS:000430730300012 |
Publisher | BIOSCIENTIFICA LTD |
The Source to Article | WOS |
Scopus ID | 2-s2.0-85044422364 |
Fulltext Access | |
Citation statistics | |
Document Type | Journal article |
Collection | Faculty of Health Sciences |
Corresponding Author | Frigo, Daniel E. |
Affiliation | 1.Center for Nuclear Receptors and Cell Signaling, University of Houston, Houston, Texas, USA 2.Department of Biology and Biochemistry, University of Houston, Houston, Texas, USA 3.Department of Cancer Systems Imaging, The University of Texas M.D. Anderson Cancer Center, Houston, Texas, USA 4.The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, Houston, Texas, USA 5.Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, USA 6.Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA 7.Dan L. Duncan Cancer Center, Baylor College of Medicine, Houston, Texas, USA 8.Biology and Pharmacology, Genome Institute of Singapore, A*STAR, Singapore, Singapore 9.Faculty of Health Sciences, University of Macau, Taipa, Macau, China 10.Department of Genitourinary Medical Oncology, The University of Texas M.D. Anderson Cancer Center, Houston, Texas, USA 11.Molecular Medicine Program, The Houston Methodist Research Institute, Houston, Texas, USA |
Recommended Citation GB/T 7714 | White, Mark A.,Tsouko, Efrosini,Lin, Chenchu,et al. GLUT12 promotes prostate cancer cell growth and is regulated by androgens and CaMKK2 signaling[J]. ENDOCRINE-RELATED CANCER, 2018, 25(4), 453-469. |
APA | White, Mark A.., Tsouko, Efrosini., Lin, Chenchu., Rajapakshe, Kimal., Spencer, Jeffrey M.., Wilkenfeld, Sandi R.., Vakili, Sheiva S.., Pulliam, Thomas L.., Awad, Dominik., Nikolos, Fotis., Katreddy, Rajasekhara Reddy., Kaipparettu, Benny Abraham., Sreekumar, Arun., Zhang, Xiaoliu., Cheung, Edwin., Coarfa, Cristian., & Frigo, Daniel E. (2018). GLUT12 promotes prostate cancer cell growth and is regulated by androgens and CaMKK2 signaling. ENDOCRINE-RELATED CANCER, 25(4), 453-469. |
MLA | White, Mark A.,et al."GLUT12 promotes prostate cancer cell growth and is regulated by androgens and CaMKK2 signaling".ENDOCRINE-RELATED CANCER 25.4(2018):453-469. |
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