Determination of aesculin concentration in rabbit pasma by RP-HPLC with fluorescence detector

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	Determination of aesculin concentration in rabbit pasma by RP-HPLC with fluorescence detector
	Chen X.-P.2; Wang Y.-Y.1; Wang M.1; Wu Q.1; Shi J.-S.1; Du G.-H.2
	2007
Source Publication	Chinese Pharmacological Bulletin
ISSN	10011978
Volume	23 Issue:4 Pages:552-554
Abstract	Aim: To establish a HPLC-FLD method for determining the concentration of aesculin in rabbit plasma. Methods: The sample was separated with a C column (150 mm x 4.6 mm, 5 μm) at 30°C with the mobile phase of methanol and water (74:26); A fluorescence detector was used (λex = 230 nm λem = 460 nm); The flow rate was 1 ml·min. Results: The retention time of aesculin was (3.620 ± 0.05) min; A good linear relationship of aesculin in the plasma samples at the concentration between 0.1 mg·L and 100 mg·L was observed (r = 0.997); The mean absolute recovery was 74.0%-82.6% and the lowest detective limit was 0.05 ng; Both the intra-day and inter-day precisions were less than 9.0%. After a single intravenous injection of aesculin (5 mg·kg) in rabbits, the T and T were (0.196 ± 0.008)·h and (6.70 ± 0.645)· h. Conclusions: The method was simple, sensitive and credible and could be used for the pharmacokinetic study of aesculin.
Keyword	Aesculin Pharmacokinetics RP-HPLC-FLD
URL	View the original
Language	英語English
Fulltext Access	View Full-Text via Scopus
Document Type	Journal article
Collection	University of Macau
Affiliation	1.Zunyi Medical College 2.Chinese Academy of Medical Sciences
Recommended Citation GB/T 7714	Chen X.-P.,Wang Y.-Y.,Wang M.,et al. Determination of aesculin concentration in rabbit pasma by RP-HPLC with fluorescence detector[J]. Chinese Pharmacological Bulletin, 2007, 23(4), 552-554.
APA	Chen X.-P.., Wang Y.-Y.., Wang M.., Wu Q.., Shi J.-S.., & Du G.-H. (2007). Determination of aesculin concentration in rabbit pasma by RP-HPLC with fluorescence detector. Chinese Pharmacological Bulletin, 23(4), 552-554.
MLA	Chen X.-P.,et al."Determination of aesculin concentration in rabbit pasma by RP-HPLC with fluorescence detector".Chinese Pharmacological Bulletin 23.4(2007):552-554.

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