| Residential College | false |
| Status | 已發表Published |
| Study on the diterpenoid constituents and anticancer action of Pteris semipinnata | |
| Zhang X.; Li J.; He C. | |
| 1999-08-01 | |
| Source Publication | Chinese Pharmaceutical Journal
 |
| ISSN | 10012494 |
| Volume | 34Issue:8Pages:512-514 |
| Abstract | OBJECTIVE: To investigate the diterpenoid constituents and anticancer action of the pteris semipinnata L. (PSL). METHOD: The ethanol extract was fractionated over an active charcoal column, eluted with ethanol-chloroform (10:1). After concentrating, the remainder was chromatographed on sillica gel column, eluted with chloroform-methanol (90:2). The pure compounds were obtained. Their structures were confirmed by spectra, espectially by two dimensional nuclear magnetic resonance (2D-NMR) for their stereo structures. Cell viability was determined using the 3-(4,5-Dimethyl-2-thia-zolyl)2,5-diphenyl-2H-tetrazolium bromide (MTT) test, and the IC value were calculated by the modified Karber formula. RESULTS: Five compounds, B, 4F, 5F, 6F and A, were obtained. A is a new compuound, 6F was isolated from the plant for the first time. A and 6F exhibited obvious activity to inhibit the growth of human promyelocytic leukemia cells (HL-60), gastric adenocarcinom cell line (MGC-803), human nasopharyngeal carcinoma cells in low differentiation (CNE-2Z) and human liver adenocarcinoma cell line (BEL-7402), 6F is more active than the positive control by 5Fu, their IC for LC-60 were 0.09 μmol.L and 52.5 μmol.L, respectively. CONCLUSION: The diterpenoids obtained from the PSL possess obvious antitumour activity. The preliminary study on the structural-activity relationship suggested that the cytotoxic activity is related to the α-methylene cyclopentanone moity. The 6F is the most active one. |
| Keyword | Antitumor Diterpenoid Ent-kaurane Pteris semipinnata L. |
| URL | View the original |
| Language | 英語English |
| Fulltext Access | |
| Document Type | Journal article |
| Collection | University of Macau |
| Affiliation | Guangdong Medical University |
| Recommended Citation GB/T 7714 | Zhang X.,Li J.,He C.. Study on the diterpenoid constituents and anticancer action of Pteris semipinnata[J]. Chinese Pharmaceutical Journal, 1999, 34(8), 512-514. |
| APA | Zhang X.., Li J.., & He C. (1999). Study on the diterpenoid constituents and anticancer action of Pteris semipinnata. Chinese Pharmaceutical Journal, 34(8), 512-514. |
| MLA | Zhang X.,et al."Study on the diterpenoid constituents and anticancer action of Pteris semipinnata".Chinese Pharmaceutical Journal 34.8(1999):512-514. |
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