The effect of compound 6F isolated from Pteris Semipinnata L on cell cycle and synthesis of DNA, RNA and protein of lung adenocarcinoma cell

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	The effect of compound 6F isolated from Pteris Semipinnata L on cell cycle and synthesis of DNA, RNA and protein of lung adenocarcinoma cell
	Li J.-H.; Liang N.-C.; Mo L.-E.; He C.-W.; Zhang X.
	1999
Source Publication	Chinese Pharmacological Bulletin
ISSN	10011978
Volume	15 Issue:1 Pages:49-51
Abstract	AIM: To study the effects of compound 6F, one of the diterpenoids from Pteris Semipinnata L on cell cycle and the synthesis of DNA, RNA and protein of lung adenocarcinoma cell line SPC-A-1. METHODS: Cell viability was determined by using 3- (4,5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2H- tetrazolium bromide (MTT) test, the cell cycle was analysed by flow cytometry (FCM) and the synthesis of DNA, RNA and protein was observed by the [H]- TdR, [H]-UTP and [H]-Leu incorporation. RESULTS: Compound 6F strongly inhibited the growth of SPC-A-1 cell, the IC value was 2.34 μmol · L after 24 h treatment. FCM analysis revealed that 6F could increase the percentage of cells in G/M phase and decrease those in G/G phase after 24 h treatment, and in a dose-dependent manner, while those in S phase changed a little. The assays of [H]-TdR [H]-UTP and [H]-Leu incorporation into DNA, RNA and protein respectively showed obvious inhibition dose-dependently after exposure to 6F for 24 h. CONCLUSION: Compound 6F remarkably inhibited SPC-A-1 cell line proliferation, arrested SPC-A-1 cell in G/M phase and inhibited the synthesis of DNA, RNA and protein.
Keyword	Cell cycle Cytotoxicity Diterpenoid Human lung adenocarcinoma cells Pteris Semipinnata L
URL	View the original
Language	英語English
Fulltext Access	View Full-Text via Scopus
Document Type	Journal article
Collection	University of Macau
Affiliation	Guangdong Medical University
Recommended Citation GB/T 7714	Li J.-H.,Liang N.-C.,Mo L.-E.,et al. The effect of compound 6F isolated from Pteris Semipinnata L on cell cycle and synthesis of DNA, RNA and protein of lung adenocarcinoma cell[J]. Chinese Pharmacological Bulletin, 1999, 15(1), 49-51.
APA	Li J.-H.., Liang N.-C.., Mo L.-E.., He C.-W.., & Zhang X. (1999). The effect of compound 6F isolated from Pteris Semipinnata L on cell cycle and synthesis of DNA, RNA and protein of lung adenocarcinoma cell. Chinese Pharmacological Bulletin, 15(1), 49-51.
MLA	Li J.-H.,et al."The effect of compound 6F isolated from Pteris Semipinnata L on cell cycle and synthesis of DNA, RNA and protein of lung adenocarcinoma cell".Chinese Pharmacological Bulletin 15.1(1999):49-51.

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