| Status | 已發表Published |
| In-minutes polymerase chain reaction with specific DNA amplification on digital microfluidics with sloppy temperature control | |
Wan, L.; Li, H. ; Chen, T.; Dong, C.; Jia, Y.; Mak, P. I.; Martins, R. P.
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| 2018-06-24 | |
| Source Publication | E-proceedings
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| Abstract | Digital microfluidics (DMF) is an emerging technology to manipulate individual microliter- to nanoliter-sized droplets on an array of electrodes by electro-wetting force [1]. Its features of electric driving and miniature footprint render it a promising technology for point-of-care diagnostics. Polymerase chain reaction (PCR) is the kernel of molecular biology and diagnostics. This temperature- dependent technique amplifies DNA in an exponential way by cycles of heating and cooling. A typical PCR often takes 1-2 h in a bulky thermal cycler. In clinical point-of-care diagnostics, reaction time and specificity of the reaction are vital to clinical decisions. Many ultra-fast PCR methods have been developed [2], exploiting the miniaturization feature of microfluidics or capillary reactors. However, they still need large accessories to support the heating and cooling process. |
| Keyword | digital microfluidics |
| Language | 英語English |
| The Source to Article | PB_Publication |
| PUB ID | 38423 |
| Document Type | Conference paper |
| Collection | DEPARTMENT OF GLOBAL LEGAL STUDIES |
| Recommended Citation GB/T 7714 | Wan, L.,Li, H.,Chen, T.,et al. In-minutes polymerase chain reaction with specific DNA amplification on digital microfluidics with sloppy temperature control[C], 2018. |
| APA | Wan, L.., Li, H.., Chen, T.., Dong, C.., Jia, Y.., Mak, P. I.., & Martins, R. P. (2018). In-minutes polymerase chain reaction with specific DNA amplification on digital microfluidics with sloppy temperature control. E-proceedings. |
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