| Residential College | false |
| Status | 已發表Published |
| Automated in vitro selection to obtain functional oligonucleotides. | |
| Zhang H.; Hamasaki A.; Toshiro E.; Aoyama Y.; Ito Y. | |
| 2000 | |
| Source Publication | Nucleic acids symposium series
 |
| ISSN | 02613166 |
| Issue | 44Pages:219-220 |
| Abstract | In vitro selection or systematic evolution of ligand by exponential enrichment (SELEX) has been devised for the identification of high-affinity oligonucleotide aptamers to target molecules. However, the selection process is repetitive and time-consuming. We have developed an automatic for in vitro selection by assembling an affinity chromato-column, a PCR thermal cycler, a HPLC and a sample operation system. Several molecular biology methods were optimized for the machine. Automated selection was used to generate nucleic acid aptamers interacting specifically with an environmental contaminant. |
| URL | View the original |
| Language | 英語English |
| Fulltext Access | |
| Document Type | Journal article |
| Collection | University of Macau |
| Affiliation | Tokushima University |
| Recommended Citation GB/T 7714 | Zhang H.,Hamasaki A.,Toshiro E.,et al. Automated in vitro selection to obtain functional oligonucleotides.[J]. Nucleic acids symposium series, 2000(44), 219-220. |
| APA | Zhang H.., Hamasaki A.., Toshiro E.., Aoyama Y.., & Ito Y. (2000). Automated in vitro selection to obtain functional oligonucleotides.. Nucleic acids symposium series(44), 219-220. |
| MLA | Zhang H.,et al."Automated in vitro selection to obtain functional oligonucleotides.".Nucleic acids symposium series .44(2000):219-220. |
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