Fractionation and molecular weight distribution of biologically active poly[3-(3,4-dihydroxyphenyl) glyceric acid] (PDPGA)-preparation from Symphytum asperum were carried out. Molecular weights and contents of different fractions for PDPGA-preparation were simultaneously determined using high performance size exclusion chromatography (HPSEC) coupled with multi angle laser light scattering (MALLS) and refractive index detector (RID) with the refractive index increment (dn/dc). Results suggested that HPSEC-MALLS-RID with the dn/dc method could be used as a routine method for quality evaluation of PDPGA-preparations from the species of genera Symphytum, Anchusa, Cynoglossum and Borago of Boraginaceae family on the content of biologically active principle PDPGA, polysaccharides impurities and standardization of PDPGA-preparations. Results may contribute to the rational usage of PDPGA-preparations from several species of Boraginaceae family S. asperum, S. caucasicum, S. officinale, S. grandiflorum, Anchusa italica, Cynoglossum officinale and Borago officinalis and are beneficial to improve the quality control of PDPGApreparations.