Benzothiazole (BTH) is an aromatic heterocyclic compound with wide industrial applications. In view of its toxicity and wide environmental presence, previous efforts have been made to decompose BTH via different degradation pathways. However, due to its recalcitrant nature, conventional biological treatment methods cannot completely degrade BTH in the wastewater. In this study, sulfate radical-based advanced oxidation process (AOP) technique has been adopted to degrade BTH in aqueous phase. Persulfate (PS) was employed as radical promotor to generate sulfate radical via heat activation. Degradation of BTH by thermally activated persulfate via AOP has been experimentally evaluated in a systematic manner. Laboratory efforts have been made to examine the impact of a number of physiochemical parameters including the type of oxidants, reaction temperature, initial concentrations of PS and BTH, solution pH, and the presence of anionic species. It shows that a higher BTH degradation rate can be achieved by lowering BTH initial concentration or increasing PS concentration. Increasing solution pH or the presence of 10 mM of Cl, Br, (Formula presented.), or (Formula presented.) species can decrease BTH degradation rate. Furthermore, the primary radical(s) responsible for BTH degradation have been identified as sulfate radical at an acidic aqueous condition, and hydroxyl radical and sulfate radical combined at a basic condition. This study provides the necessary theoretical and technical foundations for BTH degradation via sulfate radical-based AOP technique. The conclusions from this study can substantially promote the field application of AOP, especially sulfate radical-based AOP technique, for BTH degradation in wastewater treatment process.