Triptolide inhibits cytokine-induced activation of retro-ocular fibroblasts from patients with graves' ophthalmopathy

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	Triptolide inhibits cytokine-induced activation of retro-ocular fibroblasts from patients with graves' ophthalmopathy
	Yan S.-X.1; Wang Y.1; Bin Z.2; Zhi-Hai F.1; Jiu-Sheng L.1; Ya-Ru S.1; Yan-Jie L.1
	2011-10-30
Source Publication	Journal of Medicinal Plant Research
ISSN	19960875
Volume	5 Issue:24 Pages:5789-5795
Abstract	To explore the effects of Triptolide which has immunosuppressive and anti-inflammatory properties on the expression of human leucocyte antigen-DR (HLA-DR), intercellular adhesion molecule-1 (ICAM-1), CD40 and Fas enduced by cytokines and the synthesis of hyaluronic acid (HA) on cultured retro-ocular fibroblasts (RFs) from patients with Graves' ophthalmopathy. Cultured RFs were incubated with IL-1 (100 U/mL) or TNF-α (100 U/mL) in the presence of Triptolide. Flow cytometry was used to investigate the amount of HLA-DR, ICAM-1, CD40 and Fas. HA synthesis was measured by radioimmunoassay. At base conditions, the percentage of positive cells of HLA-DR, ICAM-1 and CD40 on RFs were 6.70 ± 3.06%, 14.89 ± 11.67% and 5.29 ± 3.02%, and the synthesis of HA was 337.8 ± 42.7 ng/ml. IL-1 or TNF-α significantly enhanced the amount of HLA-DR, ICAM-1 and CD40 and HA synthesis. Triptolide 0.01 μg/L had little effect on cytokine- induced HLA-DR, ICAM-1 and CD40 amounts, as well as HA synthesis. When the concentration ranged from 0.1 to 10 μg/L, Triptolide inhibited cytokine- induced RFs activation in a dose-dependent manner. Triptolide itself also induced Fas expression on RFs in a dose-dependent manner. Triptolide could inhibit cytokines-induced activation of RFs derived from patients with Graves' ophthalmopathy. © 2011 Academic Journals.
Keyword	Cytokine Fibroblasts Graves'ophthalmopathy Retro-ocular Triptolide
URL	View the original
Language	英語English
Fulltext Access	View Full-Text via Scopus
Document Type	Journal article
Collection	University of Macau
Affiliation	1.First Affiliated Hospital of Zhengzhou University 2.Henan College of Traditional Chinese Medicine
Recommended Citation GB/T 7714	Yan S.-X.,Wang Y.,Bin Z.,et al. Triptolide inhibits cytokine-induced activation of retro-ocular fibroblasts from patients with graves' ophthalmopathy[J]. Journal of Medicinal Plant Research, 2011, 5(24), 5789-5795.
APA	Yan S.-X.., Wang Y.., Bin Z.., Zhi-Hai F.., Jiu-Sheng L.., Ya-Ru S.., & Yan-Jie L. (2011). Triptolide inhibits cytokine-induced activation of retro-ocular fibroblasts from patients with graves' ophthalmopathy. Journal of Medicinal Plant Research, 5(24), 5789-5795.
MLA	Yan S.-X.,et al."Triptolide inhibits cytokine-induced activation of retro-ocular fibroblasts from patients with graves' ophthalmopathy".Journal of Medicinal Plant Research 5.24(2011):5789-5795.

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