AN GENE ENGINF; ERING RECOMBINANT ANTI-CEA, ANTI-CD3 AND ANTI-CD28 SINGLE-CHAIN TRI-SPECIFIC ANTIBODY | ANTICORPS TRISPECIFIQUE MONOCATENAIRE RECOMBINE ANTI-CEA, ANTI-CD3 ET ANTI-CD28, MIS AU POINT GENETIQUEMENT

Patent Number	WO2005095456A1
Status	申請中 Pending
	AN GENE ENGINF; ERING RECOMBINANT ANTI-CEA, ANTI-CD3 AND ANTI-CD28 SINGLE-CHAIN TRI-SPECIFIC ANTIBODY \| ANTICORPS TRISPECIFIQUE MONOCATENAIRE RECOMBINE ANTI-CEA, ANTI-CD3 ET ANTI-CD28, MIS AU POINT GENETIQUEMENT
Application Number	WOCN05000408
Application Date	2005-03-29
Rights Holder	DONGGUAN HAOFA BIOTECHNOLOGY DEVELOPMENT \| BEIJING ABT GENETIC ENG TECHNOLOGY CO LTD
	WANG, Xiangbin; HUANG, Hualiang; ZHAO, Baofeng; ZHAO, Qi; PIAO, Jinhua; LIN, Qing
Date Available	2005-10-13
Country	World intellectual property organization
Abstract	This present invention is related to a recombinant single-strand tri-specific antibody made from antitumor antibody, FC interlinker, anti-human CD3 antibody, HSA interlinker and anti-human CD28 antibody in turn. Particularly, the invention relates to an anti-CEA, anti-CD3 and anti-CD28 recombinant single-strand tri-specific antibody: C A-TsAb, which was constructed with three tandem antibody fragments (anti-CEA scFv, anti-CD3 scFv and anti-CD28 single-domain antibody) linked by two interlinkers (FC interlinker and HSA interlinker), and could be appended by c myc tag or histidine tag ((His) 6-tag) in the C termination. It also concerns a metlhod for construction, expression and purification of the antibody. It also offers the encoded DNA sequence of the antibody, expression vectors and host cells for the vectors. \| L'invention concerne un anticorps trispécifique monocaténaire recombiné, fait à partir d'un anticorps antitumoral, d'un fragment Fc d'un anticorps humain anti-CD3, d'un fragment HSA et d'un anticorps humain anti-CD28. L'invention concerne en particulier CEA-TsAb, un anticorps trispécifique monocaténaire recombiné anti-CEA, anti-CD3 et anti-CD28, formé de trois fragments d' anticorps en tandem (scFv anti-CEA, scFv anti-CD3 et anticorps anti-CD28 à domaine unique) liés par deux fragments (fragments Fc et HSA), et auquel une étiquette c-myc ou histidine ((His) 6) peut être ajoutée à l'extrémité C. L'invention concerne aussi un procédé de construction, d'expression et de purification de l'anticorps ; et la séquence d'ADN codée de l'anticorps, des vecteurs d'expression et des cellules hôtes pour les vecteurs.
Language	中文Chinese
Open (Notice) Number	WO2005095456A1
IPC Classification Number	C07K16/18 ; C12N15/13 ; C12N15/63 ; C12N15/70 ; A61K39/395 ; A61P35/00 ; C07K16/28 ; C07K16/30 ; C07K19/00 ; C12N15/12
CPC Classification Number	A61K2039/505 ; A61P35/00 ; A61P43/00 ; C07K16/00 ; C07K16/2809 ; C07K16/2818 ; C07K16/3007 ; C07K2317/34 ; C07K2317/626 ; C07K2317/73
Agency	YU SARN AUDREY & PARTNERS
Document Type	Patent
Collection	University of Macau
Recommended Citation GB/T 7714	WANG, Xiangbin,HUANG, Hualiang,ZHAO, Baofeng,et al. AN GENE ENGINF; ERING RECOMBINANT ANTI-CEA, ANTI-CD3 AND ANTI-CD28 SINGLE-CHAIN TRI-SPECIFIC ANTIBODY \| ANTICORPS TRISPECIFIQUE MONOCATENAIRE RECOMBINE ANTI-CEA, ANTI-CD3 ET ANTI-CD28, MIS AU POINT GENETIQUEMENT. WO2005095456A1.
APA	WANG, Xiangbin., HUANG, Hualiang., ZHAO, Baofeng., ZHAO, Qi., PIAO, Jinhua., & LIN, Qing (2005-03-29). AN GENE ENGINF; ERING RECOMBINANT ANTI-CEA, ANTI-CD3 AND ANTI-CD28 SINGLE-CHAIN TRI-SPECIFIC ANTIBODY \| ANTICORPS TRISPECIFIQUE MONOCATENAIRE RECOMBINE ANTI-CEA, ANTI-CD3 ET ANTI-CD28, MIS AU POINT GENETIQUEMENT.

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