Objective: To evaluate the effect of iron on the stability of fat emulsion in total nutrient admixture (TNA) in pediatrics. Methods: Based on intravenous nutrition preparation and handling protocols, 0, 0.25, 0.50, 0.75, and 1.00 mg iron sucrose was added in each 100 ml TNA, respectively, and each dose was prepared for 10 bags. The stability of fat emulsion was observed by electron scanning microscopy. The pH and osmolality were determined at different time points (0, 24, 48, and 72 h). Results: The fat particle size, percentages of particles > 0.5 μm, pH value, and osmolality were not significantly different among all the groups at different time points (all P>0.05). The mean particle sizes of the fat emulsion were <0.5 μm in all groups within 72 h. No fat particle with diameter >5 μm was seen in each group. Conclusion: TNA containing iron sucrose with concentrations range 0.25-1.00 mg per 100 ml is stable.