Two Novel Dermaseptin-Like Antimicrobial Peptides with Anticancer Activities from the Skin Secretion of Pachymedusa dacnicolor

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	Two Novel Dermaseptin-Like Antimicrobial Peptides with Anticancer Activities from the Skin Secretion of Pachymedusa dacnicolor
	Shi , D.; Hou , X.; Wang , L.; Gao , Y.; Wu , D.; Xi, X.; Zhou, M.; Kwok, H. F.; Duan , J.; Chen, T.; Shaw, C.
	2016-05-12
Source Publication	Toxins
ISSN	2072-6651
Pages	144---
Abstract	The dermaseptin antimicrobial peptide family contains members of 27–34 amino acids in length that have been predominantly isolated from the skins/skin secretions of phyllomedusine leaf frogs. By use of a degenerate primer in Rapid amplification of cDNA ends (RACE) PCR designed to a common conserved domain within the 5′-untranslated regions of previously-characterized dermaseptin encoding cDNAs, two novel members of this peptide family, named dermaseptin-PD-1 and dermaseptin-PD-2, were identified in the skin secretion of the phyllomedusine frog, Pachymedusa dacnicolor. The primary structures of both peptides were predicted from cloned cDNAs, as well as being confirmed by mass spectral analysis of crude skin secretion fractions resulted from reversed-phase high-performance liquid chromatography. Chemically-synthesized replicates of dermaseptin-PD-1 and dermaseptin-PD-2 were investigated for antimicrobial activity using standard model microorganisms (Gram-positive bacteria, Gram-negative bacteria and a yeast) and for cytotoxicity using mammalian red blood cells. The possibility of synergistic effects between the two peptides and their anti-cancer cell proliferation activities were assessed. The peptides exhibited moderate to high inhibition against the growth of the tested microorganisms and cancer cell lines with low haemolytic activity. Synergistic interaction between the two peptides in inhibiting the proliferation of Escherichia coli and human neuronal glioblastoma cell line, U251MG was also manifested.
Keyword	amphibian peptide antimicrobial anticancer molecular cloning
URL	View the original
Language	英語English
The Source to Article	PB_Publication
PUB ID	16927
Document Type	Journal article
Collection	DEPARTMENT OF BIOMEDICAL SCIENCES Faculty of Health Sciences Cancer Centre
Corresponding Author	Kwok, H. F.
Recommended Citation GB/T 7714	Shi , D.,Hou , X.,Wang , L.,et al. Two Novel Dermaseptin-Like Antimicrobial Peptides with Anticancer Activities from the Skin Secretion of Pachymedusa dacnicolor[J]. Toxins, 2016, 144---.
APA	Shi , D.., Hou , X.., Wang , L.., Gao , Y.., Wu , D.., Xi, X.., Zhou, M.., Kwok, H. F.., Duan , J.., Chen, T.., & Shaw, C. (2016). Two Novel Dermaseptin-Like Antimicrobial Peptides with Anticancer Activities from the Skin Secretion of Pachymedusa dacnicolor. Toxins, 144---.
MLA	Shi , D.,et al."Two Novel Dermaseptin-Like Antimicrobial Peptides with Anticancer Activities from the Skin Secretion of Pachymedusa dacnicolor".Toxins (2016):144---.

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