Triggering of cancer cell cycle arrest by a novel scorpion venom-derived peptide -- Gonearrestide

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	Triggering of cancer cell cycle arrest by a novel scorpion venom-derived peptide -- Gonearrestide
	Li, B.; Lyu, P.; Xi, X.; Mahadevappa, R.; Ge, L.; Shaw, C.; Kwok, H. F.
	2018-06-01
Source Publication	Journal of Cellular and Molecular Medicine
ISSN	1582-4934
Pages	DOI:10.1111/jcmm.13745---
Abstract	In this study, a novel scorpion venom-derived peptide named Gonearrestide was identified in an in-house constructed scorpion venom library through a combination of high throughput NGS transcriptome and MS/MS proteome platform. In total, 238 novel peptides were discovered from two scorpion species; and 22 peptides were selected for further study after a battery of functional prediction analysis. Following a series of bioinformatics analysis alongside with in vitro biological functional screenings, Gonearrestide was found to be a highly potent anticancer peptide which acts on a broad spectrum of human cancer cells while causing few if any observed cytotoxic effects on epithelial cells and erythrocytes. We further investigated the precise anticancer mechanism of Gonearrestide by focusing on its effects on the colorectal cancer cell line, HCT116. NGS RNA-sequencing was employed to obtain full gene expression profiles in HCT116 cells, cultured in the presence and absence of Gonearrestide, in order to dissect signaling pathway differences. Taken together the in vitro, in vivo and ex vivo validation studies, it was proven that Gonearrestide could inhibit the growth of primary colon cancer cells and solid tumors by triggering cell cycle arrest in G1 phase through inhibition of cyclin-dependent kinases 4 (CDK4) and up-regulate the expression of cell cycle regulators/inhibitors - cyclin D3, p27 and p21. Furthermore, prediction of signaling pathways and potential binding sites used by Gonearrestide are also presented in this study.
Keyword	Venom library NGS transcriptome MS/MS proteome Anticancer mechanism Signaling pathways Binding sites
Language	英語English
The Source to Article	PB_Publication
PUB ID	36317
Document Type	Journal article
Collection	DEPARTMENT OF BIOMEDICAL SCIENCES
Corresponding Author	Kwok, H. F.
Recommended Citation GB/T 7714	Li, B.,Lyu, P.,Xi, X.,et al. Triggering of cancer cell cycle arrest by a novel scorpion venom-derived peptide -- Gonearrestide[J]. Journal of Cellular and Molecular Medicine, 2018, DOI:10.1111/jcmm.13745---.
APA	Li, B.., Lyu, P.., Xi, X.., Mahadevappa, R.., Ge, L.., Shaw, C.., & Kwok, H. F. (2018). Triggering of cancer cell cycle arrest by a novel scorpion venom-derived peptide -- Gonearrestide. Journal of Cellular and Molecular Medicine, DOI:10.1111/jcmm.13745---.
MLA	Li, B.,et al."Triggering of cancer cell cycle arrest by a novel scorpion venom-derived peptide -- Gonearrestide".Journal of Cellular and Molecular Medicine (2018):DOI:10.1111/jcmm.13745---.

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