| Residential College | false |
| Status | 已發表Published |
| Rapid discrimination of colon cancer cells with single base mutation in KRAS gene segment using laser tweezers Raman spectroscopy | |
Liu, M.; Liu, X.; Huang, Z.; Tang, X.; Lin, X.; Xu, Y.; Kwok, H. F.  ; Lin, Y.; Feng, S.
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| 2018-11-28 | |
| Source Publication | Journal of Biophotonics
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| ISSN | 1864-0648 |
| Pages | ----- |
| Abstract | Laser tweezers Raman spectroscopy (LTRS) as a label-free and non-invasive technology has been proven to be an ideal tool for analysis of living cells which provides important fingerprint information without interference from surrounding environments. In this study, LTRS system was successfully employed to trap the colon cancer cells with different KRAS mutation status, including DKS-8 (KRAS wild-type) and DLD-1 (KRAS mutant-type), HKE-3 (KRAS wild-type) and HCT-116 (KRAS mutant-type), and further obtained their corresponding Raman spectra. Changes of some vital biomolecules due to the gene mutation state were sensitively recorded by our home-made LTRS system. As a result of the comparison between DKS-8 and DLD-1 cells, an index of 97.5% of correct classification was obtained by combining LTRS with PCA-LDA statistical analysis, while an index of 97.0% of correct classification was achieved between HKE-3 and HCT-116 cells. Moreover, between mutant-type cells (DKS-8 and HKE-3) versus wild-type cells, the index of correct classification was 81.2%, which was considered good. Our preliminary results showed that the LTRS system coupled with PCA-LDA analysis will have a great potential for further applications in the rapid and label-free detection of circulating tumor cells in liquid biopsy. |
| Keyword | Raman optical tweezers KRAS mutant Cancer cell screening |
| URL | View the original |
| Language | 英語English |
| The Source to Article | PB_Publication |
| PUB ID | 42391 |
| Document Type | Journal article |
| Collection | DEPARTMENT OF BIOMEDICAL SCIENCES Faculty of Health Sciences Cancer Centre |
| Corresponding Author | Kwok, H. F.; Lin, Y.; Feng, S. |
| Recommended Citation GB/T 7714 | Liu, M.,Liu, X.,Huang, Z.,et al. Rapid discrimination of colon cancer cells with single base mutation in KRAS gene segment using laser tweezers Raman spectroscopy[J]. Journal of Biophotonics, 2018, -----. |
| APA | Liu, M.., Liu, X.., Huang, Z.., Tang, X.., Lin, X.., Xu, Y.., Kwok, H. F.., Lin, Y.., & Feng, S. (2018). Rapid discrimination of colon cancer cells with single base mutation in KRAS gene segment using laser tweezers Raman spectroscopy. Journal of Biophotonics, -----. |
| MLA | Liu, M.,et al."Rapid discrimination of colon cancer cells with single base mutation in KRAS gene segment using laser tweezers Raman spectroscopy".Journal of Biophotonics (2018):-----. |
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