Isolation of cytoplasmatic proteins from cultured cells for two-dimensional gel electrophoresis.

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Status	已發表Published
	Isolation of cytoplasmatic proteins from cultured cells for two-dimensional gel electrophoresis.
	Wang Y.; Chiu J.F.; He Q.Y.
	2008-06-05
Source Publication	Methods in molecular biology (Clifton, N.J.)
ISSN	10643745
Volume	425 Pages:101-112
Abstract	Cytoplasma is the cell interior place between the cellular membrane and the nucleus, where various intracellular activities take place, including energy production, reactive oxygen species (ROS) detoxification, heme synthesis, nitrogen and lipid metabolism, phosphorylation in signal transduction, and cytoskeletal meshwork construction. The rich cytoplasmatic proteins carrying out these intracellular functions are interesting targets for biochemical and molecular biological studies. The relatively recent discipline of proteomics offers a chance to globally analyze the changes in cytoplasmic proteins corresponding to drug treatments or disease conditions, and thus provide target candidates for further biological validation in drug development and biomarker discovery. Isolation of cytoplasmic proteins from cells is a necessary step for high resolution protein separation by two-dimensional gel electrophoresis (2DE) and specific proteomic analysis.
URL	View the original
Language	英語English
Fulltext Access	View Full-Text via Scopus
Document Type	Journal article
Collection	University of Macau
Affiliation	The University of Hong Kong
Recommended Citation GB/T 7714	Wang Y.,Chiu J.F.,He Q.Y.. Isolation of cytoplasmatic proteins from cultured cells for two-dimensional gel electrophoresis.[J]. Methods in molecular biology (Clifton, N.J.), 2008, 425, 101-112.
APA	Wang Y.., Chiu J.F.., & He Q.Y. (2008). Isolation of cytoplasmatic proteins from cultured cells for two-dimensional gel electrophoresis.. Methods in molecular biology (Clifton, N.J.), 425, 101-112.
MLA	Wang Y.,et al."Isolation of cytoplasmatic proteins from cultured cells for two-dimensional gel electrophoresis.".Methods in molecular biology (Clifton, N.J.) 425(2008):101-112.

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