Objective: The objective of this study was to investigate the correlation between health-related fitness (body-composition, muscular endurance, muscular strength, flexibility and cardiovascular fitness) and the cognition of sports knowledge among College Students in Macau. Methodology: The targeted participants of this study were 104 university students (41 boys and 63 girls) in Macau, aged from 19 to 21. Various fitness data was collected and a cognitive theory assessment was adopted to examine the students in different cognitive results. Descriptive statistics, oneway ANOVA, correlational study and regression investigation were carried out in this study for data analysis. Conclusion: This study found that health-related fitness and sports knowledge showed significant correlation especially in lower stage domains in the cognitive theory. In regression models, it showed that health-related fitness has predictive impact over cognition of sports knowledge for both male and female, in particular with female. It would be better to encourage students to improve their health-related fitness to achieve better cognitive results.