Using a sequential mixed-methods approach, this study explored how knowledge hiding (KH) targets react to perpetrators in the hospitality industry. Study 1 entailed in-depth interviews of 20 employees from 13 hotels. Findings indicate that KH influences knowledge transfer behavior through negative emotions. Moreover, individual personality and motivation, team and interpersonal factors, job characteristics, and KH characteristics can moderate this effect. In Studies 2 (n = 54) and 3 (n = 118), two scenario-based experiments reveal that KH negatively affects targets’ knowledge sharing (KS) with perpetrators directly and indirectly through negative emotions. In Study 4, the results of a survey from 475 employees indicate that when the need for affiliation of employees and task interdependence is high, the negative effect of negative emotions on KS with perpetrators is weaker. This study advances the literature on KH by offering a sound theoretical treatment of emotional concerns and the interaction between knowledge seekers and knowledge hiders.