In this study, a non-contact liquid crystal thermography (LCT) method was developed for surface temperature measurements on the micro-and micro-tubes with the diameters of 2000μm, 1000μm, 838μm and 686μm. In the single-phase heat transfer experiments, the fully-developed flow heat transfer data from laminar to turbulent regionswere obtained under the uniform wall heat flux boundary condition. The experiment system was verified with the macro-tube of 2000μm diameter tube. The results showed that buoyancy effect was not observed in the laminar region when the tube diameter was less than 1000 μm. In theturbulent region, the heat transfer data trend followed with the classical turbulent correlation. Furthermore, the current LCT results showed the similar trend when compare with the published LCT data of Yang and Lin[1]. © (2013) Trans Tech Publications, Switzerland.