Researchers have increasingly paid attention to prejudice against trans people and its predictors. However, few studies have explored if potential predictors may interact to affect people’s attitudes towards trans people. In this experimental study, we examined the impacts of participant-related factors (i.e. gender and prior contact experience with gender or sexual minorities), target-related variables (i.e. gender identity and facial appearance), and their interactions on Chinese young adults’ attitudes towards trans men. We recruited 172 Chinese young adults to rate images of men with different gender identity labels (i.e. cisgender or trans men) and facial appearances (i.e. feminine or masculine). Results showed that a three-way interaction existed among targets’ gender identity labels, targets’ facial appearances, and participants’ genders. Overall, female participants were less likely than men to show more negative attitudes towards trans men than cisgender men. Participants showed more positive attitudes towards masculine-appearing trans men than feminine trans men. The current findings implied that people favoured trans men’s masculine faces, which match the roles of their gender identities.