This study investigated the acute changes in glycemic homeostasis in response to brief, high-intensity, intermittent exercise in obese adults. Ten obese adults (age: 25.3±5.1 years; body mass index: 32.0±4.0) volunteered to participate in the study. The time-course changes in blood glucose in response to a 75-g oral glucose tolerance test were examined following: high-intensity intermittent exercise (HIE) of four 30-second all-out cycling efforts interspersed with 4-minute active recovery periods; 24 hours of recovery post-HIE (REC); and no treatment (control, CON). Blood glucose in each trial was measured before, and 30, 60, 90, and 120 minutes after glucose intake (GI). Blood glucose (mmol/L) at 30 minutes post-GI in the HIE (6.9±0.4) trial was significantly lower (p<0.05) than the corresponding values of the CON (9.0±0.4) and REC (8.8±0.4) trials. Blood glucose concentration at 120 minutes post-GI in the REC (5.7±0.3) trial was also significantly lower (p<0.05) than the corresponding value of the CON (6.9±0.4) trial. When blood glucose values were plotted against corresponding time points, the area under the curve (mmol/L/minute) of the CON (890±43) trial was significantly greater (p<0.05) than that of the HIE (834±40) and REC (846±32) trials. The HIE and REC conditions were not significantly different (p>0.05). This study demonstrated the improvement in glycemic homeostasis in obese adults immediately after brief, high-intensity, intermittent exercise. These effects were maintained for at least 24 hours postexercise. The findings may be useful in the development of time-efficient lifestyle intervention strategies for improving obesity-related risk factors for diabetes.