Enhancing the professional knowledge in different levels of operator is a critical success factor to advance the performance of manufacturing industry. However, the traditional training system is lack of scientific method to transfer the professional knowledge (tacit knowledge) to the operator. Applied RFID and Virtual Reality Technology in Knowledge-based Training System can convert the tacit knowledge to the explicit knowledge to different levels of operator. The trainee can capture the basic operation skill through the web-enabled and knowledge-based training system. Moreover, the system can provide the working experience and operation history about the production and tool application to the trainee through RFID technology. They can quickly and conveniently search their target tools that will apply the real manufacturing processes without any human-being supervising. The cost of training resource can be saved, because the workload of supervisor or senior operation is reduced. The senior operation can report the updating and real-time situation of production and tools through RFID technology with knowledge-based training system. The closed loop knowledge can enhance the precision level of analysis results of production system. The industry can be sustainable development through this system. ©2010 IEEE.