Precise Droplet Splitting on Digital Microfluidic Chip with Blade Structures

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	Precise Droplet Splitting on Digital Microfluidic Chip with Blade Structures
	Dong, C.; Jia, Y.; Chen, T.; Gao, J.; Wan, L.; Mak, P. I.; Vai, M. I.; Martins, R. P.
	2016-10-13
Source Publication	MicroTAS 2016
Abstract	Parallelization and miniaturization are always the goals of molecular diagnostics for disease detection from the minute amount of available samples. For the goal of parallelization in digital microfluidics, a uniform on-chip droplet splitting is highly demanded. The volume variation and binary splitting nature of the current splitting practices prevents the use of DMF applications on precise multiple analyses of one single drop. To address this issue, here we introduce a new approach to enhance droplet splitting by integrating micro structures, which is so called blades, on the DMF chips. Daughter droplet volume variation observed in experiments is reduced while applying this method to a various kinds of fluids. The droplet separating ratio (20% to 80%) can be accurately controlled by tactfully locate the blade position. In a single splitting process, two to five equal volume daughter droplets can be generated by multiple blades fabricated on one electrode. Additionally, by applying this technique to a sepsis pathogen detection assay, fast, quantitative and multiple DNA detection can be achieved in one step.
Keyword	digital microfluidics microstructure
Language	英語English
The Source to Article	PB_Publication
PUB ID	30679
Document Type	Conference paper
Collection	Faculty of Education
Recommended Citation GB/T 7714	Dong, C.,Jia, Y.,Chen, T.,et al. Precise Droplet Splitting on Digital Microfluidic Chip with Blade Structures[C], 2016.
APA	Dong, C.., Jia, Y.., Chen, T.., Gao, J.., Wan, L.., Mak, P. I.., Vai, M. I.., & Martins, R. P. (2016). Precise Droplet Splitting on Digital Microfluidic Chip with Blade Structures. MicroTAS 2016.

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